色胺
化学
色谱法
色氨酸
质谱法
氘
化学电离
选择性离子监测
气相色谱法
5-羟基色氨酸
血清素
离子
分析化学(期刊)
气相色谱-质谱法
电离
有机化学
生物化学
氨基酸
受体
物理
量子力学
作者
Tokishi Hayashi,Michio Shimamura,Fumio Mizutani,Yukiko Minatogawa,Hiroshi Naruse,Yoshio Iida
出处
期刊:Journal of Chromatography B: Biomedical Sciences and Applications
[Elsevier]
日期:1986-01-01
卷期号:383: 259-269
被引量:23
标识
DOI:10.1016/s0378-4347(00)83472-6
摘要
Sensitive methods for the determination of deuterated and non-deuterated tryptophan, tryptamine and serotonin by combined capillary gas chromatography and negative ion chemical ionization mass spectrometry were developed. [3,3-2H2]-l-Tryptophan, which was used as a tracer, was synthesized for studies of their in vivo metabolism. Tryptophan was converted into its trifluoroacetylmethyl derivative after prepurification with an AG 50W-X2 cation-exchange column. Tryptamine and serotonin were extracted with 20% butanol in diethyl ether and derivatized with trifluoroacetic anhydride. These derivatives were separated and determined by selected ion monitoring. In these determinations, [2′,3,3,4′,5′,6′,7′-2H7]-d,l-tryptophan, [α,α,β,β-2H4]tryptamine and [α,α,β,β-2H4]serotonin were used as internal standards.
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