过敏原
大蠊
蟑螂
重组DNA
原肌球蛋白
生物
免疫球蛋白E
美洲蟑螂
免疫印迹
过敏
德国蟑螂
分子生物学
微生物学
免疫学
生物化学
抗体
基因
肌动蛋白
生态学
作者
J A Asturias,N Gómez-Bayón,Maite Arilla,Alberto Martı́nez,Ricardo Palácios,F Sánchez-Gascón,Jorge Martı́nez
出处
期刊:Journal of Immunology
[The American Association of Immunologists]
日期:1999-04-01
卷期号:162 (7): 4342-4348
被引量:130
标识
DOI:10.4049/jimmunol.162.7.4342
摘要
Inhalation of allergens produced by the American cockroach (Periplaneta americana) induces IgE Ab production and the development of asthma in genetically predisposed individuals. The cloning and expression in Escherichia coli of P. americana tropomyosin allergen have been achieved. The protein shares high homology with other arthropod tropomyosins (80% identity) but less homology with vertebrate ones (50% identity). The recombinant allergen was produced in E. coli as a nonfusion protein with a yield of 9 mg/l of bacterial culture. Both natural and recombinant tropomyosins were purified by isoelectric precipitation. P. americana allergen 1 (Per a 1) and Per a 7 (tropomyosin) are to date the only cross-reacting allergens found in cockroaches. ELISA and Western blot inhibition experiments, using natural and recombinant purified tropomyosins from shrimp and cockroach, showed that tropomyosin induced cross-reactivity of IgE from patients allergic to these allergens, suggesting that this molecule could be a common allergen among invertebrates.
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