白细胞清除术
转导(生物物理学)
细胞毒性T细胞
免疫系统
淋巴因子激活杀伤细胞
细胞疗法
生物
嵌合抗原受体
遗传增强
免疫学
白细胞介素12
癌症研究
出处
期刊:Methods in molecular biology
日期:2022-01-01
卷期号:: 311-328
标识
DOI:10.1007/978-1-0716-2160-8_21
摘要
Autologous T cells expressing chimeric antigen receptor (CAR) have produced a spectacular response in hematological malignancies. This success of cellular therapy has inspired the exploration of the therapeutic potential of other immune cell types. In this regard, natural killer (NK) cells hold great potential as they can identify tumor cells by mechanisms that are different from those used by T cells and have a high cytotoxic capacity. Their capacity to recognize tumors and killing potency can be further enhanced by genetic modification. Our laboratory has developed a clinically adaptable method to manufacture genetically modified NK cells using retroviral vectors in compliance with current good manufacturing practice regulations. Here, we describe relevant technical procedures, including isolation of peripheral blood mononucleated cells from a leukapheresis product, T-cell depletion, stimulation and transduction of NK cells, and preparation of transduced NK cells for infusion.
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