色谱法
毛细管电泳
化学
基质(化学分析)
电动现象
固相萃取
吸附剂
萃取(化学)
检出限
色散(光学)
电解质
石蒜碱
分析化学(期刊)
生物碱
吸附
物理
有机化学
物理化学
光学
电极
立体化学
作者
Chu Chu,Linmin Lian,Gao Wei,Yaping Zang,Yanfang Zou,Yangbin Lv,Chenyue Li,Shengqiang Tong,Jizhong Yan,Suhong Chen
标识
DOI:10.1016/j.microc.2022.107314
摘要
An efficient off-line and on-line synergistic preconcentration strategy which involves combining micro matrix solid-phase dispersion (MSPD) with electrokinetic supercharging (EKS) in capillary zone electrophoresis (CZE) has been developed for the determination of two alkaloids, lycorine and galanthamine, in complicated matrix samples. This method was comprehensively optimized in terms of the buffer condition, the leading electrolyte (LE), terminating electrolyte (TE), the sample injection in EKS preconcentration, as well as the dispersing sorbent, the sample/dispersing sorbent mass ratio, grinding time, eluting solvent in MSPD procedure. Under the optimum conditions, lycorine and galanthamine, achieved about 1500- and 1200-fold sensitivity improvement respectively when compared to the ordinary injection. A satisfactory correlation coefficient (R2 > 0.9982) was obtained in the range of 10–500 ng/mL. The limits of detection (LODs) for lycorine and galanthamine were 6.0 ng/mL and 8.0 ng/mL, respectively. Moreover, the proposed method was successfully applied in two real samples, rat fecal samples and Bulbus Lycoridis Radiatae, to demonstrate its feasibility. Recoveries in spiked fecal and plant samples were 89.51% to 107.4%. The result showed that the new developed method possessed the great potential in analyzing trace alkaloids in complicated samples matrix.
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