水飞蓟宾
上皮-间质转换
生物
癌症研究
卵巢癌
波形蛋白
活力测定
癌细胞
细胞凋亡
PI3K/AKT/mTOR通路
蛋白激酶B
体内
细胞生长
癌症
免疫学
转移
生物化学
免疫组织化学
遗传学
作者
Narges Maleki,Negar Yavari,Maryam Ebrahimi,Ahmad Faisal Faiz,Roya Khosh Ravesh,Aysan Sharbati,Mohammad Panji,Keivan Lorian,Abdollah Gravand,Mojtaba Abbasi,Omid Abazari,Mehdi Shafiee Mehr,Yasin Eskandari
出处
期刊:Gene
[Elsevier]
日期:2022-05-01
卷期号:823: 146275-146275
被引量:10
标识
DOI:10.1016/j.gene.2022.146275
摘要
Silibinin, the principal flavonoid derived from milk thistle seeds, has been demonstrated to have strong inhibitory effects against human malignancies. The inhibitory function of silibinin on ovarian cancer, however, is not fully identified. In this essay, both in vivo and in vitro investigations were conducted to survey the silibinin's blocking effects on ovarian cancer.The impacts of silibinin on two ovarian cancer cell lines, SKOV-3 and A2870, were determined by evaluating cell viability, migration, invasion, and apoptosis. Q-RT-PCR and western blotting techniques were carried out to explore the protein levels of signaling pathway markers. A mouse xenograft model was utilized to determine the silibinin efficacy in inhibiting tumor growth.After cell treatment with silibinin, cell viability, migration, and invasion were appreciably inhibited in cancer cell lines, but cell apoptosis was promoted. Also, silibinin reversed the epithelial-mesenchymal transition (EMT) mechanism by inducing E-cadherin expression and reducing N-cadherin and vimentin expression, suppressing the levels of regulators related to EMT such as Snail, Slug, and ZEB1 transcription factors, and also decreasing PI3K/AKT, Smad2/3, and β-catenin intermediate molecules in vitro. Silibinin effectively ameliorated tumor growth in vivo.silibinin could be considered a potent agent against ovarian cancer based on the results.
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