Molecular structure and evolution characteristics of dissolved organic matter in groundwater near landfill: Implications of the identification of leachate leakage

Landfills can cause groundwater contamination, the pollution characteristics in groundwater near landfill sites have been extensively investigated, while the rapid identification of leachate leakage remained unclear. Comprehensively characterizing dissolved organic matter (DOM) is crucial for tracing the source, species, and migration of contaminants within groundwater and protecting groundwater sources. Here, we showed that DOM composition from newer landfills was mainly composed of newly-produced tryptophan and tyrosine, and protein-like and humic-like substances were more abundant in landfills that were relatively older. DOM in landfill groundwater was initially dominated by outputs from microbial activities, followed by terrigenous input. Leaked leachate contained an additional dye-derived fluorescent matter at the excitation/emission wavelength of 240–260/440–460 nm that was absent in uncontaminated groundwater. Leachate leakage increased the concentrations of humic-like substance, DOM molecular weight, and microbial activity in the downstream groundwater, resulting in the microorganisms rapidly multiply and secrete large amounts of microbial metabolism by-products, making them suitable indicators of groundwater pollution. Three criteria were proposed to establish an interpretable fluorescence method to identify leachate pollution. The obtained results provide a novel insight into not only the monitoring, early warning, and identification but also the transport, fate and removal or transformation of groundwater leachate in landfills. • Changes in groundwater-derived DOM related to leachate leakage were analyzed. • Leachate leakage increased microbial activity, DOM humification and molecular weight. • Leachate-contaminated groundwater contained dye-derived fluorescent matter. • Interpretable fluorescence method to identify leachate pollution was developed.