抗体
药代动力学
新生儿Fc受体
细胞内
化学
细胞培养
受体
细胞
单克隆抗体
药理学
免疫球蛋白G
生物
免疫学
生物化学
遗传学
作者
Chang Liu,Yeon-Su Kim,John Lowe,Shan Chung
出处
期刊:Bioanalysis
[Newlands Press Ltd]
日期:2021-07-01
卷期号:13 (14): 1135-1144
被引量:4
标识
DOI:10.4155/bio-2021-0099
摘要
Aim: Evaluation of suitable pharmacokinetic properties is critical for successful development of IgG-based biotherapeutics. The prolonged half-lives of IgGs depend on the intracellular trafficking function of neonatal Fc receptor, which rescues internalized IgGs from lysosomal degradation and recycles them back to circulation. Results: Here, we developed a novel cell-based assay to quantify recycling of monoclonal antibodies in a transwell culture system that uses a cell line that stably expresses human neonatal Fc receptor. We tested seven therapeutic antibodies and showed that the recycling output of the assay strongly correlated with the clearance in humans. Conclusion: This recycling assay has potential application as a pharmacokinetic prescreening tool to facilitate development and selection of IgG-based candidate therapeutic monoclonal antibodies.
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