穗
玉米素
生物
分生组织
细胞分裂素
水稻
开枪
基因敲除
转基因水稻
葡萄糖基转移酶
分蘖(植物学)
细胞生物学
植物
农学
基因
转基因
转基因作物
生长素
遗传学
作者
Xiaoling,Shang Shang,Rong-Rong,Weibo Xie,Hua Hua,Tian Tian,Qing-long,Hong Wang,Fang-Qing,Youguang Guo
出处
期刊:植物学报:英文版
日期:2016-01-01
卷期号: (7): 627-641
摘要
As a ubiquitous reaction, glucosylation controls the bioactivity of cytokinins in plant growth and development.Here we show that genetic manipulation of zeatin-Oglucosylation regulates the formation of important agronomic traits in rice by manipulating the expression of OscZOG1 gene,encoding a putative zeatin O-glucosyltransferase. We found that OscZOG1 was preferentially expressed in shoot and root meristematic tissues and nascent organs. The growth of lateral roots was stimulated in the overexpression lines, but inhibited in RNA interference lines. In shoots, knockdown of OscZOG1 expression by RNA interference significantly improved tillering, panicle branching, grain number per panicle and seed size, which are important agronomic traits for grain yield. In contrast, constitutive expression of OscZOG1 leads to negative effects on the formation of the grain-yielding traits with a marked increase in the accumulation levels of cis-zeatin O-glucoside(c ZOG) in the transgenic rice plants. In this study,our findings demonstrate the feasibility of improving the critical yield-determinant agronomic traits, including tiller number, panicle branches, total grain number per panicle and grain weight by downregulating the expression level of OscZOG1. Our results suggest that modulating the levels of cytokinin glucosylation can function as a fine-tuning switch in regulating the formation of agronomic traits in rice.
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