线粒体
化学
细胞生物学
线粒体DNA
细胞
细胞器
细胞凋亡
细胞质
肝癌
癌细胞
生物
程序性细胞死亡
线粒体内膜
作者
Yaschar Kabiri,Carola Eberhagen,Sabine Schmitt,Percy A. Knolle,Hans Zischka
出处
期刊:Methods of Molecular Biology
日期:2021-01-01
卷期号:2277: 277-287
被引量:1
标识
DOI:10.1007/978-1-0716-1270-5_17
摘要
Isolation of mitochondria is a crucial method for examining molecular details of this organelle's manifold functions. Historically, mitochondrial isolations required large amounts of sample material which impeded their isolation from cultured cells. We have therefore developed a method allowing for controlled and reproducible isolation of intact and functional mitochondria from diverse cell types in culture. Here we provide a methodological update of this approach together with a protocol for the subsequent analysis of such isolated mitochondria by electron microscopy. Combining the isolation procedure with this powerful imaging method can reveal ultrastructural mitochondrial peculiarities in disease settings that might not be evident in intact cells and allows for assessment of mitochondrial membrane integrity and sample purity.
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