炎症体
髓鞘碱性蛋白
烟酰胺腺嘌呤二核苷酸磷酸
吡喃结构域
神经炎症
目标2
NADPH氧化酶
化学
半胱氨酸蛋白酶1
白细胞介素18
免疫学
内分泌学
内科学
分子生物学
生物化学
髓鞘
医学
氧化应激
生物
氧化酶试验
炎症
受体
细胞因子
酶
中枢神经系统
作者
Ya’nan Qi,Zhibao Guo,Xiang-En Meng,Yan Liu,Shuyi Pan,Dazhi Guo
出处
期刊:Undersea & Hyperbaric Medicine
[Undersea and Hyperbaric Medical Society (UHMS)]
日期:2020-10-01
卷期号:: 607-619
被引量:3
标识
DOI:10.22462/10.12.2020.10
摘要
Neuroinflammation plays an important role in brain damage after acute carbon monoxide poisoning (ACOP). The nucleotide-binding domain, leucine-rich-containing family, pyrin domain-containing (NLRP) 3 inflammasome triggers the activation of inflammatory caspases and maturation of interleukin (IL)-1β and -18, and has been linked to various human autoinflammatory and autoimmune diseases. In this study we investigated the effects of hyperbaric oxygen (HBO2) on NLRP3 inflammasome activation after ACOP. Mice were randomly divided into four groups: sham group (exposure to normobaric air – i.e., 21% O2 at 1 atmosphere absolute); HBO2-only group; CO + normobaric air group; and CO + HBO2 group. Cognitive function was evaluated with the Morris water maze; myelin injury was assessed by Fluoro-Myelin GreenTM fluorescent myelin staining and myelin basic protein (MBP) immunostaining; and mRNA and protein levels of NLRP3 inflammasome complex proteins were measured by quantitative real-time PCR and Western blot, respectively. Additionally, serum and brain levels of IL-1β and -18 and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase were determined by enzyme-linked immunosorbent assay. It was found that HBO2 improved learning and memory, and alleviated myelin injury in mice subjected to acute CO exposure. Furthermore, HBO2 decreased NLRP3, absent in melanoma 2 (AIM2), caspase-1, and apoptosis-associated speck-like protein containing a C-terminal caspase recruitment domain mRNA and protein levels, and reduced brain and serum concentrations of IL-1β and -18 and NADPH oxidase. These results indicate that HBO2 suppresses the inflammatory response after ACOP by blocking NLRP3 inflammasome activation, thereby alleviating cognitive deficits.
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