Simultaneous Detection of Intracellular Nitric Oxide and Peroxynitrite by a Surface-Enhanced Raman Scattering Nanosensor with Dual Reactivity

A functional surface-enhanced Raman scattering (SERS) nanosensor which can simultaneously detect nitric oxide (NO) and peroxynitrite (ONOO–) in living cells is explored. The SERS nanosensor is fabricated through modifying gold nanoparticles (AuNPs) with newly synthesized 3,4-diaminophenylboronic acid pinacol ester (DAPBAP), which has two reactive groups. The simultaneous detection achieved in this work is not only because of the SERS spectral changes of the nanosensor resulting from the dual reactivity of DAPBAP on AuNPs with NO and ONOO– but also by the narrow SERS bands suitable for multiplex detection. Owing to the combination of SERS fingerprinting information and chemical reaction specificity, the nanosensor has great selectivity for NO and ONOO–, respectively. In addition, the nanosensor has a wide linearity range from 0 to 1.0 × 10–4 M with a submicromolar sensitivity. More importantly, simultaneous monitoring of NO and ONOO– in the Raw264.7 cells has been fulfilled by this functional nanosensor, which shows that the SERS strategy will be promising in comprehension of the physiological issues related with NO and ONOO–.