多糖
体内
化学
硫酸化
体外
剂量
药理学
谷胱甘肽
生物化学
生物
酶
生物技术
作者
Cui Liu,Cheqing Jin,Entao Li,Qin Fan,Deyun Wang,Peng Li,Xiuping Li,Xingying Chen,Shulei Qiu,Zhenzhen Gao,Hongquan Li,Yuanliang Hu
标识
DOI:10.1016/j.intimp.2014.12.023
摘要
Codonopsis pilosula polysaccharide (CP) was extracted, purified and modified by chlorosulfonic acid-pyridine method to obtain a sulfated CP (sCP). Their antioxidative activities in vitro were compared through the free radical-scavenging test. The results demonstrated that the scavenging capabilities of sCP were significantly stronger than those of CP. In vivo test, the mice hepatic injury model was prepared by BCG/LPS method, then administrated respectively with sCP and CP at three dosages, the biochemical indexes in serum, antioxidative indexes in liver homogenate and histopathological change in liver of the mice were compared. The results showed that in high (200 mg/kg) and middle (150 mg/kg) dosages of sCP groups, the contents of ALT, AST and TNF-α in serum and MDA in liver homogenate were significantly lower than those in the model group and numerically lower than those in the CP groups, the activities of SOD and GSH-Px in liver homogenate were significantly higher than those in the model group and numerically higher than those in the CP groups. In the model group there were obvious pathological changes in the liver, while in the sCP groups were near normal. These results indicate that sCP and CP possess antioxidative activity in vitro and in vivo, the activity of sCP is stronger than that of CP and sulfation modification can enhance the antioxidative and hepatoprotective activities of Codonopsis pilosula polysaccharide.
科研通智能强力驱动
Strongly Powered by AbleSci AI