软骨
材料科学
生物医学工程
耳廓
纳米纤维素
组织工程
复合材料
纤维素
解剖
化学
医学
有机化学
作者
Luc Nimeskern,Héctor Martínez Ávila,Johan Sundberg,Paul Gatenholm,Ralph Müller,Kathryn S. Stok
标识
DOI:10.1016/j.jmbbm.2013.03.005
摘要
Bacterial nanocellulose (BNC) is a novel non-degradable biocompatible material that promotes chondrocyte adhesion and proliferation. In this work, its potential use in ear cartilage tissue engineering (TE) is investigated. Firstly, the mechanical properties of native ear cartilage are measured in order to set a preliminary benchmark for ear cartilage replacement materials. Secondly, the capacity of BNC to match these requirements is assessed. Finally, a biofabrication process to produce patient-specific BNC auricular implants is demonstrated. BNC samples (n=78) with varying cellulose content (2.5–15%) were compared using stress-relaxation indentation with human ear cartilage (n=17, from 4 males, aged 49–93 years old). Additionally, an auricle from a volunteer was scanned using a 3T MRI with a spoiled gradient-echo sequence. A negative ear mold was produced from the MRI data in order to investigate if an ear-shaped BNC prototype could be produced from this mold. The results show that the instantaneous modulus Ein, equilibrium modulus Eeq, and maximum stress σmax of the BNC samples are correlated to effective cellulose content. Despite significantly different relaxation kinetics, the Ein, Eeq and σmax of BNC at 14% effective cellulose content reached values equivalent to ear cartilage (for Eeq, BNC: 2.4±0.4 MPa and ear cartilage: 3.3±1.3 MPa). Additionally, this work shows that BNC can be fabricated into patient-specific auricular shapes. In conclusion, BNC has the capability to reach mechanical properties of relevance for ear cartilage replacement, and can be produced in patient-specific ear shapes.
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