A series of monodispersed P(St-MMA-AA) colloids with diameters from 200 nm to 500 nm was synthesized successfully using emulsion polymerization. The corresponding colloidal crystals were fabricated and investigated. UV-Vis reflectance spectra showed that the band gaps of the obtained colloidal crystals accord well with Bragg law. The height, phase and spectrum 2D images of the obtained binary colloidal crystals were studied using different modes of atom force microscopy.