小发夹RNA
A549电池
体内
腺癌
癌症研究
细胞生长
体外
细胞周期
生物
化学
细胞
细胞生物学
细胞培养
癌症
内科学
医学
基因敲除
生物化学
生物技术
遗传学
作者
Seung‐Ho Jang,Seon Young Choi,Pan Dong Ryu,So Yeong Lee
标识
DOI:10.1016/j.ejphar.2010.10.066
摘要
Voltage-gated potassium (Kv) channels are widely expressed in the plasma membranes of numerous cells and contribute to a variety of cellular functions in both excitable neuronal cells and non-excitable epithelial cells. Recently, it has been demonstrated that Kv channels are associated with the proliferation of several types of cancer cells. In the present study, we investigated the effects of suppression of Kv1.3 expression on cell proliferation and cell cycle progression in human lung adenocarcinoma, A549 cells. Treatment with margatoxin (MgTX), a selective blocker of Kv1.3 or short hairpin RNA (shRNA) against Kv1.3, significantly blocked A549 cells' proliferation. In addition, selective inhibition of Kv1.3 significantly increased expression level of p21(Waf1/Cip1) and significantly decreased the expression level of Cdk4 and cyclin D3. We also applied the MgTX into a xenograft model using nude mice, and MgTX caused a reduction of tumor volume when it was injected into the tumor tissues. These results suggest that Kv1.3 may serve as a novel therapeutic target for lung adenocarcinoma therapy.
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