Rapid HPLC of Cyanidin and Delphinidin of an Anthocyanin Complex Exposed to Human Gingival Epithelial Cells

A rapid isocratic HPLC was developed and validated for use in simultaneous analysis of cyanidin and delphinidin extracted from purple cobs of Z ea mays L. ceritina Kulesh. (CC), blue petals of Clitoria ternatea L. (CT) and an anthocyanin complex (AC). The method was shown to be rapid, precise and accurate within 5 20 μg/ml ( r > 0.997) with limits of detection and quantitation of 0.45 and 1.52 μg/ml for cyanidin and 4.04 and 13.3 μg/ml for delphinidin, respectively. It could quantitatively detect and compare changes in cyanidin and delphinidin from the AC exposed to human gingival epithelium cells.