Isolation and characterization of NAD(P)-dependent L-sorbosone dehydrogenase from Gluconobacter melanogenus UV10.

NAD(P)-Dependent L-sorbosone dehydrogenase has been purified from the cytosol fraction of Gluconobacter melanogenus UV10.The enzyme was purified about 30-fold with an overall yield of 19%by column chromatographies on DEAE-Sepharose CL-6B, DEAE-SephadexA-50, and Blue Sepharose CL-6B.The enzyme required NADor NADPas a co factor, and showed broad substrate specificity on various aldehyde compounds.Amongthem, L-sorbosone was the best substrate for the enzyme.In this respect, the enzyme is a kind of aldehyde dehydrogenase.