LipidBlast in silico tandem mass spectrometry database for lipid identification

Lipid Blast is an in silico–generated tandem mass spectral library covering more than 119,000 lipid compounds from 26 different classes, providing a useful tool for lipid identification in metabolomics studies. Current tandem mass spectral libraries for lipid annotations in metabolomics are limited in size and diversity. We provide a freely available computer-generated tandem mass spectral library of 212,516 spectra covering 119,200 compounds from 26 lipid compound classes, including phospholipids, glycerolipids, bacterial lipoglycans and plant glycolipids. We show platform independence by using tandem mass spectra from 40 different mass spectrometer types including low-resolution and high-resolution instruments.