High speed fluorescence photoactivation localization microscopy imaging

Imaging live biological samples to study biomolecular dynamics requires a very high spatial and temporal resolution. Superresolution localization microscopy has allowed researchers to investigate biological systems whose sizes are below the diffraction limit (200-250 nm) using an optical microscope. Fluorescence Photoactivation Localization Microscopy (FPALM) and other localization microscopy techniques have recently been shown to be capable of rendering superresolution images obtained with acquisitions of shorter than 0.5 seconds. Here we will discuss the FPALM imaging technique, at both lower and higher imaging speeds. This talk will focus on the advantages, challenges, and drawbacks of high speed imaging localization microscopy.