Cloning and Sequencing of VP1 Gene of Feline Parvovirus CC-1 Strain

To analyze the sequence of VP1 gene of Feline parvovirus CC-1 strain and compare with that of representative virus strain.According to the reported complete nucleotide sequence of FPV(CU-4) in GenBank,a pair of primers were designed and synthesized.VP1 gene of isolate was amplified.The products of PCR were cloned into PGM-T vector.the positive clone was identified bu enzyme cutting,PCR and sequencing.The whole sequence of VP1gene was analyzed using DNAStar software.The results indicated that the VP1 gene with 2306 bp long had a single opening read frame 2184 bp in length and coded a polypeptide of 727 amino acids.The sequence analysis demonstrated that represent reference strains loaded down from GenBank,the result of ORF sequence analysis indicated that CC-1 strain exhibited 99.3% homology of the nucleotides and amino acid with that of the XJ-1 strain in our county.To analyze the sequence of VP1 full gene,have a significance in clinic and epidemiological study.