Objective To discuss the prevention and the therapeutic means of the iatrogenic bile duct injuries.Methods The clinical data of 36 patients with the iatrogenic bile duct injuries were reviewed retrospectively.Result 41 operations were performed in 36 cases.among them,2 times operation were carried out in 5 cases.The last surgical treatment was performed in all patients by the end to end anastomosis of bile duct in 2 cases,the repair of bile duct injury in 4 ones,the removal of suture in 3 ones,the Roux-en-Y choledochojejunostomy in 27 ones.The data from 28 cases who was followed up from 1~8 years showed 90% patients presented good results.Conclusion The main cause of iatrogenic bile duct injuries was choledcystectomy,the bile duct injuries can be prevented.The Roux-en-Y choledochojejunostomy assroach was the best choice in the reconstruction of iatrogenic biliary tract injuries and traumatic stricture of bile duct.