Chemical constituents from stems and leaves of Cryptolepis buchananii

Objective To study the chemical constituents from the stems and leaves of Cryptolepis buchananii. Methods Compounds were isolated and purified by chromatography on silica gel, Sephadex LH-20, ODS columns, and preparative HPLC. Their structures were identified by NMR and MS, as well as comparison on spectral data with literature values. Results Thirteen compounds were obtained from the EtOAc fraction of methanol extract in the stems and leaves of C. buchananii and their structures were elucidated as isoscopoletin(1),(+)-3-hydroxy-β-ionone(2),(3R, 6R, 7E)-3-hydroxy-4, 7-megastigmadien-9-one(3), ficusic acid(4),(+)-pinoresinol(5),(+)-8-hydroxypinoresinol(6),(+)-syringaresinol(7), diaaurantiamide acetate(8), loliolide(9),(–)-balanophonin(10), chrysoeriol(11), 9-hydroxy-10E, 12Z-octadecadienoic acid methyl ester(12), and ficusesquilignan A(13). Conclusion All the compounds are isolated from the plants of Cryptolepis R. Br. for the first time.