Inhibitory effects of volatile compounds from fresh petals of Rosa rugosa 'Zifurong',R.rugosa 'Saixizi',R.rugosa 'Xizi',R.rugosa 'Purple Branch',R.rugosa 'Puce Dragon' 5 Rosa rugosa cultivars were investigated on bacteria and fungi in the air.The results showed that the 5 Rosa rugosa cultivars exerted inhibitory effects on bacteria and fungi differently.The analysis indicated that the ability of inhibiting the growth of bacteria and fungi had highly significant differences among the 5 Rosa rugosa cultivars(F=8.615,P=0.0001),and 3 different bloom stages(F=5.871,P=0.0071).The 5 Rosa rugosa cultivars investigated all possessed inhibitory effect and the effect of R.rugosa 'Purple Branch' was the best and changed less in the whole bloom season than others.The antibacterial rate had highly significant differences among the 5 Rosa rugosa cultivars(F=13.405,P=0.0001),and 3 different bloom stages(F=6.512,P=0.0045).Among the 5 cultivars,R.rugosa 'Purple Branch',R.rugosa'Xizi' and R.rugosa'Zifurong' had stronger antibacterial activity in the whole bloom season.The differences of the antifungal rate among the 5 cultivars and 3 different bloom stages were not significant.In fully bloom stage,R.rugosa'Purple Branch',R.rugosa'Puce Dragon' and R.rugosa'Zifurong' were found most effective in inhibiting bacteria,while with R.rugosa'Purple Branch' and R.rugosa'Saixizi' in fungi.