Association between angiogenic factors and signs of arterial aging in women with pre‐eclampsia

ABSTRACT Objective Pre‐eclampsia ( PE ) is associated with an increased risk of cardiovascular disease later in life. In cases with PE there is a substantial increase in levels of the antiangiogenic factor soluble fms‐like tyrosine kinase‐1 ( sFlt ‐1) and decreased levels of the proangiogenic factor placental growth factor ( PlGF ). Elevated levels of sFlt ‐1 are also found in individuals with cardiovascular disease. The aims of this study were to assess levels of sFlt ‐1, PlGF and the sFlt ‐1/ PlGF ratio and their correlation with signs of arterial aging by measuring the common carotid artery ( CCA ) intima and media thicknesses and their ratio (I/M ratio) in women with and without PE . Methods Serum sFlt ‐1 and PlGF levels were measured using commercially available enzyme‐linked immunosorbent assay kits, and CCA intima and media thicknesses were estimated using high‐frequency (22‐ MHz ) ultrasonography in 55 women at PE diagnosis and in 64 women with normal pregnancy at a similar gestational age, with reassessment at 1 year postpartum. Results During pregnancy, higher levels of sFlt ‐1, lower levels of PlGF , a thicker intima, a thinner media and a higher I/M ratio of the CCA were found in women with PE vs controls (all P < 0.0001). Further, sFlt ‐1 and the sFlt ‐1/ PlGF ratio were positively correlated with intima thickness and I/M ratio (all P < 0.0001). At 1 year postpartum, levels of sFlt ‐1 and the sFlt ‐1/ PlGF ratio had decreased in both groups; however, their levels in the PE group were still higher than in the controls ( P = 0.001 and < 0.0001, respectively). Levels of sFlt ‐1 and the sFlt ‐1/ PlGF ratio remained positively correlated with intima thickness and I/M ratio at 1 year postpartum. Conclusions Higher sFlt ‐1 levels and sFlt ‐1/ PlGF ratio in women with PE were positively associated with signs of arterial aging during pregnancy. At 1 year postpartum, sFlt ‐1 levels and the sFlt ‐1/ PlGF ratio were still higher in the PE group and were associated with the degree of arterial aging. © 2016 The Authors. Ultrasound in Obstetrics & Gynecology published by John Wiley & Sons Ltd on behalf of the International Society of Ultrasound in Obstetrics and Gynecology.