Objective: To study the skin irritation and curative effect of Zengxiao Ruyi Jinhuanggao. Methods: To observe the impact that the skin irritation and curative effect of Zengxiao Ruyi Jinhuanggao on healthy rabbit. Results: No irritation reaction occurred on skin after applied Zengxiao Ruyi Jinhuanggao many times and no reactions of erythema, dropsy, bleeding spots, pigmentation occurred. Conclusion: The results indicated that the drug is effective and safe, and there isn't irritation reaction on skin after applied with the drug.