Study on the adaptation changes of intestinal microbiota in Ctenopharyngodon idellus fed on different diets

PCR-DGGE fingerprinting was used to reveal the adaptation process of intestinal microbiota in Ctenopharyngodon idellus fed on artificial diet and Sudan grass. DGGE profiles of foregut,midgut and hindgut samples were compared and the results showed that the bacterial communities from hindgut contents were significant differences with those from foregut and midgut contents in C. idellus fed the same diet( TH0- F0=- 2. 268,P 0. 05; TH0- M0=- 2. 470,P 0. 05). Moreover,the bacterial communities of artificial feed and Sudan grass samples from the same intestinal segment( P 0. 05) also exhibited significant differences. In addition,Shannon index( H') of hindgut samples was almost no less than 4. 25,while H' of foregut and midgut samples was generally no more than 4. 25. The DGGE analysis of 27 hindgut samples indicated that intestinal microbiota reached to stabilization at 11 days later from feeding on sundan grass instead of artificial feed. In conclusion,microbiotas in different intestinal segments were different in C. idellus and food transformation also has a fast and significant influence on the composition of gut microbiota.