效价
载体(分子生物学)
病毒载体
第一代
病毒学
生物
第三代
计算生物学
编码(社会科学)
基因
计算机科学
病毒
医学
遗传学
数学
重组DNA
统计
人口
环境卫生
电信
出处
期刊:Humana Press eBooks
[Humana Press]
日期:2014-01-01
卷期号:: 211-229
被引量:8
标识
DOI:10.1007/978-1-62703-679-5_15
摘要
High-capacity adenoviral vectors (HC-Ad), also known as “helper-dependent” (HD-Ad), “gutless”, “gutted”, or “third-generation” Ad vectors, are devoid of all viral coding sequences and have shown promising potential for a wide variety of different applications—from classic gene therapy to genetic vaccination and tumor treatment. However, compared to first-generation adenoviral vectors their production is more complex and requires specific in-depth knowledge. This chapter delivers a detailed protocol for the successful production of HC-Ad vectors to high titers.
科研通智能强力驱动
Strongly Powered by AbleSci AI