Mechanism Involved in the Apoptosis of Human Umbilical Vein Endothelial Cells Induced by Proteasome Inhibitor MG132

Objective To study the effect of proteasome inhibitor MG132 on expression of caspase-3 and UPP associated genes in the apoptosis of human umbilical vein endothelial cells induced. Methods human umbilical vein endothelial cells were treated with MG132(2 μmol/L,5 μmol/L) for 24 hours.The apoptotic cells were detected by flow cytometric analysis.The level of E1,E2,E3 and caspase-3 mRNA expression were detected by reverse transcription-polymerase chain reaction(RT-PCR).Caspase-3 protein expression was detected by immunocytochemistry. Results The results showed that E1,E2,E3 genes expression were decreased in treatment group and apoptosis rate were increased obviously.MG132 could up-regulate the gene/protein expression of caspase-3. Conclusions Protesome inhibitor MG132 could induced human umbilical vein endothelial cells apoptosis by inhibitting UPP activity,up-regulating the gene/protein expression of caspase-3.