A Study on Accumulation Rule of Thiamphenicol in Different Tissues of Common Carp and Crucian Carp

The accumulation rule of thiamphenico (TAP) indifferent tissues of common carp (Cyprinus carpio) and crucian carp (Carassius auratus) were studied.After oral administration of TAP with adosage of 30mg/kg body weight perday for 3days,the samples were collected at 24 h afterdrug withdrawal.A quantitative analysis was performed with external stand ard method and TAP concentrations in tissues were analyzed by high performance liquid chromatography-tand em mass spectrometry (HPLC-MS/MS).The results indicated that the TAP had a good linear relationship (R2= 0.998 7) in the range of 0.1 ng/mL to 200 ng/mL concentration and the recovery rate varied from 82.37% to 107.92%.TAP showed the same accumulation trend in various tissues of fish,among which,it accumulated the highest in blood plasma and kidney and the lowest in gill and spleen.TAP was also found to accumulate in swim bladder,liver,intestine,bile,gonad,muscle and skin.In addition,the accumulation content of TAP was a little higher in ovary than in testis.Therefore,more attention should be paid to the effects of TAP on kidney and gonad to avoid thedamage to body or physiological function when TAP was applied to the prevention and control of bacterialdiseases in common carp and crucian carp.