Optimization and Comparison of Methods of Removing Eggshell Membrane and Stratum Corneum

To choose some effective methods of removing eggshell membrane and stratum corneum,motheds of EDTA,proteinase K and NaOH were optimized and compared in this paper.The observation results with SS-550 scanning electron microscope showed:firstly,the method of 5% EDTA treating eggshell 30 min could remove the inner shell membranes clearly and outer shell membranes as much as possible,but the capability of removing the stratum corneum was weaker;secondly,the method of 50 μg/mL proteinase K treating eggshell 48 h could completely remove the stratum corneum and the inner shell membranes,while the outer shell membranes could not be removed completely;thirdly,the method of 2% NaOH boiling eggshell 17 min at 99 ℃ could remove eggshell membranes and stratum corneum completely;finally,among the three methods,the method of 2% NaOH boiling eggshell 17 min at 99 ℃ showed more efficient.