Electrotransformation of Staphylococci

Methods for genetic manipulation of Staphylococci have advanced considerably over the past decade. Transduction is the most common and simplest method for transferring plasmid DNA or chromosomal markers between strains of Staphylococcus aureus. However, some clinical isolates of S. aureus may be difficult or impossible to transduce because of restriction barriers. Moreover, staphylococcal phages are highly species-specific and cannot be propagated in species other than S. aureus.