Study of degranulation in mast cell RBL-2H3 induced by Tween 80

Objective:To study directive effects of multiple kinds of injective excipients on the degranualation in rat mast cell line RBL-2H3 and to provide some indications for establishing a screening and evaluating method for pseudoallergy induced by different Tween 80.Methods:RBL-2H3 cells were cultured and treated with varying dose of Tween 80, and then the amount of releasing β-hexosaminidase was detected.MTT assay was demonstrated to determine the cytotoxicity of injective excipients which causing RBL-2H3 cells degranulation.Results:All of eight different Tween 80 products directly induced RBL-2H3 cells degranulation in a concentration-dependent manner while the degree of this effect was varying (ShanghaiWeier (oral)Weier (A)=Weier (B)=Weier (injective)=WeierNof=Croda).Tween 80 (Shanghai) showed cytotoxicity effect in a concentration-dependent manner.Conclusion:Tween 80 can induce RBL-2H3 cells degranulation directly, and the degree of effect is related to the different quality among these products.Cell degranulation induced by Tween 80 (Shanghai) dues to its cytotoxicity.