Enzyme Dynamic Inhibition of Acarbose on Type III α-Glucosidase

In this paper,the inhibitory effect of acarbose on type Ⅲ α-glucosidase( extracted from Rhizopus) was studied through3,5-dinitrosaliclic method with potato starch as a substrate. Conditions of accurate determination of the initial catalytic reaction rate of type Ⅲ α-glucosidase were found. Within 30 min the reaction rate was a liner equation curve at 40℃ and p H4. 5. When the inhibitory effect of acarbose on type Ⅲ α-glucosidase was discucced,the result of IC50= 6. 56 × 10- 6mol / L was obtained. With the double reciprocal plot method,Km= 13. 16 mmol / L of α-glucose was calculated and the inhibition type of acarbose was a hybrid of noncompetitive and competitive inhibition.