单线态氧
光化学
活性氧
化学
过氧化氢
光动力疗法
荧光
超氧化物
电子转移
生物分子
光生物学
光诱导电子转移
氧气
生物化学
有机化学
量子力学
生物
物理
酶
植物
作者
María García-Díaz,Ying‐Ying Huang,Michael R. Hamblin
出处
期刊:Methods
[Elsevier]
日期:2016-10-01
卷期号:109: 158-166
被引量:88
标识
DOI:10.1016/j.ymeth.2016.06.025
摘要
Photodynamic therapy involves the excitation of a non-toxic dye by harmless visible light to produce a long-lived triplet state that can interact with molecular oxygen to produce reactive oxygen species (ROS), which can damage biomolecules and kill cells. ROS produced by electron transfer (Type 1) include superoxide, hydrogen peroxide and hydroxyl radical (HO), while singlet oxygen (1O2) is produced by energy transfer. Diverse methods exist to distinguish between these two pathways, some of which are more specific or more sensitive than others. In this review we cover the use of two fluorescence probes: singlet oxygen sensor green (SOSG) detects 1O2; and 4-hydroxyphenyl-fluorescein (HPF) that detects HO. Interesting data was collected concerning the photochemical pathways of functionalized fullerenes compared to tetrapyrroles, stable synthetic bacteriochlorins with and without central metals, phenothiazinium dyes interacting with inorganic salts such as azide.
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