水解物
胡桃
胃蛋白酶
磺酰罗丹明B细胞培养试剂染料
化学
谷蛋白
醇溶蛋白
生物化学
癌细胞
细胞毒性
酶
分子生物学
食品科学
癌症
水解
生物
体外
贮藏蛋白
基因
遗传学
作者
Wilman Carrillo,José Ángel Gómez Ruiz,Ana Lúcia Tasca Góis Ruiz,João Ernesto de Carvalho
出处
期刊:Journal of Medicinal Food
[Mary Ann Liebert]
日期:2017-11-01
卷期号:20 (11): 1063-1067
被引量:12
标识
DOI:10.1089/jmf.2017.0063
摘要
Proteins from Juglans regia L. were isolated. Then, proteins were hydrolyzed with different enzymes. Antiproliferative activity of proteins and of the protein hydrolysates of J. regia L. were evaluated using the sulforhodamine B method. Glutelin and prolamin proteins presented a high antiproliferative activity against cancer cells PC-3 (prostate) and K-562 (leukemia) with values of 43.9 and 84.4 μg/mL, respectively. The highest inhibitory effect observed was 50% at 0.25 μg/mL concentration in gastrointestinal digestion with pepsin and corolase pp in a dose-dependent manner against cancer cell UACC62 (melanoma). Pepsin hydrolysate showed inhibitory effects against cancer cell UACC62 (melanoma) with a concentration of 71.0 μg/mL. The effects were studied in a dose-dependent manner. The hydrolysate obtained with neutrase enzyme presented inhibitory effects against cancer cell UACC62 (melanoma) at a concentration of 25 μg/mL. Neither proteins nor protein hydrolysates presented cytotoxicity against normal cell assay VERO (epithelial).
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