极性(国际关系)
荧光
溶酶体
电池极性
荧光寿命成像显微镜
生物物理学
化学
材料科学
细胞
生物化学
生物
酶
光学
物理
作者
Miao Li,Jiangli Fan,Haidong Li,Jianjun Du,Saran Long,Xiaojun Peng
出处
期刊:Biomaterials
[Elsevier]
日期:2018-05-01
卷期号:164: 98-105
被引量:87
标识
DOI:10.1016/j.biomaterials.2018.02.044
摘要
Lysosomal polarity affects the interaction activities between enzymes and substrates at the cellular level. Abnormal lysosomal polarity closely linked with disorders and diseases is worthy of attention. The first fluorescence probe, which can image polarity ratiometrically and detect lysosomal polarity quantitatively, is reported herein. The probe termed NOH can emit dual-peaks both in solvents (λem = 474, 552 nm) and in micro-environment. NOH exhibits the Boltzmann function response of the fluorescence intensity ratio to the polarity in a wide range and localizes at lysosomes specifically (Rr = 0.97). In the method of ratiometric fluorescence imaging with NOH, the variation of lysosomal polarity (Δf) can be directly discerned by the color changes. In virtue of ratiometric fluorescence imaging and the Boltzmann function relationship between the fluorescence intensity ratio and the polarity, lysosomal polarity in MCF-7 cells was calculated to be 0.224 and the polarity in the condition of lysosomal storage disorders (or cell death) could also be obtained. This probe will be a promising tool for studying lysosome-related physiological or pathological processes.
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