棘层
角蛋白
角蛋白14
角质形成细胞
角蛋白5
免疫学
角质层
桥粒蛋白
发病机制
表皮(动物学)
白细胞介素
白细胞介素13
桥粒胶蛋白3
医学
生物
病理
细胞因子
自身抗体
体外
抗体
解剖
转基因小鼠
基因
转基因
生物化学
作者
Ami Totsuka,Miyuki Omori‐Miyake,Makoto Kawashima,Junji Yagi,Yuichiro Tsunemi
出处
期刊:European Journal of Dermatology
[John Libbey Eurotext]
日期:2017-05-01
卷期号:27 (3): 247-253
被引量:39
标识
DOI:10.1684/ejd.2017.2985
摘要
In the pathogenesis of atopic dermatitis (AD), skin barrier dysfunction and T-helper (Th) type 2 immune reactions play important roles. Alterations in the stratum spinosum of AD have not been studied in much detail. In this study, we investigated the changes of structural proteins and adhesion molecules residing in the stratum spinosum of AD lesional skin, and whether Th2 cytokines including interleukin (IL)-4 and IL-13 alter the expression of these proteins. Skin samples were collected from patients with AD and from healthy controls. Normal human epidermal keratinocytes were cultured and differentiated in the presence or absence of either IL-4 or IL-13 in vitro. The expression of keratin 1, keratin 10, desmoglein 1 and desmocollin 1 was examined by immunofluorescence and western blotting. The expression of these proteins was downregulated in AD lesional skin, and IL-4 and IL-13 were shown to suppress their expression. These results suggest that the stratum spinosum is impaired in AD lesional skin, possibly by Th2 cytokines, which may be involved in the pathogenesis of AD.
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