化学
脂肪酶
生物传感器
安培法
壳聚糖
色谱法
玻璃碳
纳米颗粒
甘油
重复性
电化学
核化学
电极
循环伏安法
有机化学
纳米技术
生物化学
酶
材料科学
物理化学
作者
Aylén Di Tocco,Sebastián Noel Robledo,Yolanda Osuna,J. Sandoval-Cortez,Adrián Marcelo Granero,N. Vettorazzi,José Luis Martínez,Elda P. Segura,Anna Iliná,Marı́a Alicia Zón,Fernando Javier Arévalo,Héctor Fernández
出处
期刊:Talanta
[Elsevier]
日期:2018-12-01
卷期号:190: 30-37
被引量:42
标识
DOI:10.1016/j.talanta.2018.07.028
摘要
A very sensitive electrochemical biosensor to determine totals triglycerides (TGs) in serum samples has been developed. It is based on the electrochemical oxidation of glycerol at glassy carbon electrodes modified with magnetic nanoparticles bonded to lipase enzyme and copper oxide nanoparticles, both supported on a multiwalled carbon nanotubes/pectin dispersion. Glycerol is produced by enzymatic reaction between the TGs present in samples and the lipase immobilized. The quantification of triglycerides was performed by amperometric measurements. The proposed electrochemical biosensor improves the performance of others methods developed for the TGs quantification. The determination of TGs does not need a pretreatment of serum samples. The PLS-1 algorithm was used for the quantification of TGs. According to this algorithm, the of detection and quantification limits were from 3.2 × 10−3 g L−1 to 3.6 × 10−3 g L−1, and from 9.6 × 10−3 to 1.1 × 10−2 g L−1, respectively. The sensitivity was 1.64 × 10−6 A L g−1. The proposed electrochemical biosensor exhibited a very good performance, a stability of 20 days, very good reproducibility and repeatability, and it is presented as a very good alternative for the determination of TGs in human serum clinical samples.
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