类胡萝卜素
虾青素
角黄素
重组DNA
生物
拉伤
基因表达
基因
表达式向量
分子生物学
叶黄素
转化(遗传学)
电穿孔
表情盒
生物化学
化学
载体(分子生物学)
解剖
作者
Hans Visser,Gerhard Sandmann,Jan C. Verdoes
出处
期刊:Humana Press eBooks
[Humana Press]
日期:2005-01-01
卷期号:: 257-272
被引量:18
标识
DOI:10.1385/1-59259-847-1:257
摘要
Modification of Xanthophyllomyces dendrorhous toward a higher carotenoid synthesis will make the exploitation of X. dendrorhous as a natural source for astaxanthin more competitive. By recombinant DNA technology, the X. dendrorhous isopentenyl-pyrophosphate isomerase-encoding gene (idi) was inserted in a X. dendrorhous expression cassette that directs expression by the endogenous glyceraldehyde-3-phosphate dehydrogenase gene expression signals. X. dendrorhous transformation vector pPR2TN was used to deliver this expression cassette, by means of electroporation, into the ribosomal DNA of X. dendrorhous strain CBS 6938, which was confirmed by Southern analysis. High-performance liquid chromatography analysis of the carotenoids produced by idi-overexpressing transformant strains indicated a lower overall carotenoid formation when compared to the control strain. This finding is in contrast to that observed in bacterial systems, where enhanced expression of the idi gene increases the carotenoid content.
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