Molecular mapping of dominant male sterile gene in the Brassica napus line Shaan‐GMS by BSA‐Seq and candidate gene association analysis

Abstract Shaan‐GMS is a spontaneous dominant genic male sterile (GMS) line in Brassica napus , and its male sterility is genetically controlled by a single gene with three alleles of different dominant relationships ( Mf > Ms , Ms > ms ). Here, whole genome resequencing was performed on the homozygous ( MsMs * MfMs ) and heterozygous ( Msms * msms ) two‐type lines of Shaan‐GMS, respectively, for localizing male sterile gene. BSA‐Seq of both two‐type lines detected one candidate gene B naA08g26080D on the chromosome A08 in common. By integrating the genetic maps of Mf / Ms and Ms / ms locus, the fertility locus of Shaan‐GMS was located in the overlapping region on the A08 chromosome containing 14 genes. Among them, five genes of interesting were sequenced. As a result, two genes ( BnaA08g25920D and BnaA08g25870D ) showed DNA sequence variation among male sterile plants ( MsMs ), fertile plants ( msms ), and restorer plants ( MfMf ) of Shaan‐GMS. The candidate gene association analysis indicated that DNA variations in the BnaA08g25870D had no relationship with male fertility in a panel of 88 rapeseed lines, therefore, BnaA08g25920D is the candidate for the male sterile gene. This study lays a foundation for molecular cloning of male sterile gene in Shaan‐GMS, and the developed molecular markers and the identified restorer lines will be useful for developing new hybrid varieties based on the dominant GMS system.