生物素
共域化
荧光
免疫荧光
原位
分子生物学
化学
生物化学
细胞
细胞膜
膜
生物
抗体
物理
有机化学
量子力学
免疫学
作者
Ke Li,Chong Xu,Benhua Wang,Sulai Liu
标识
DOI:10.1016/j.saa.2024.124274
摘要
γ-Glutamyl transpeptidase (GGT), a cell-surface enzyme, is strongly implicated in mammalian malignancy growth and migration processes including human hepatocarcinogens. However, simply and conveniently detect of GGT on the cell membrane remains highly challenging. In this study, a biotin-tagged fluorescent probe Nap-biotin-glu was developed using glutamic acid, naphthalimide, and biotin as the reaction site, fluorescent reporter, and membrane-targeting group, which required only three steps. Colocalization fluorescence imaging and immunofluorescence analysis indicated that probe Nap-biotin-glu was successfully realized in situ visualizing of GGT on the cell membrane.Owing to the significant over-expressed GGT level in tumor, the probe was successfully applied to distinguish cancer tissues from adjacent normal tissues.
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