Rapid detection of eugenol in perch utilizing electrochemical method by transition metal substituted polyoxometalates

Food safety concerns caused by the application of spice allergens to fish anaesthesia. In this paper, a chitosan-reduced graphene oxide/polyoxometalates/poly-l-lysine (CS-rGO/P2Mo17Cu/PLL) modified electrode was constructed by electrodeposition and successfully applied to the quantitative analysis of eugenol (EU). The detection limit was 0.4490 μM in the linear range of 2x10-6 M to 1.4x10-5 M. It was applied to the determination of EU residues in kidney, liver and meat tissues of perch with recoveries ranging from 85.43 to 93.60%. Besides, the electrodes demonstrate high stability (2.56% drop in current value after 70 days at room temperature), high reproducibility (RSD of 4.87% for 6 parallel electrodes) and extremely fast response time. This study provided a new material for the electrochemical detection of EU.