Study on Mechanism of Huangqi-Shanyao-Danshen Compatibility in Treatment of Diabetic Nephropathy Based on GEO Database and Network Pharmacology and Molecular Docking

To explore the significance of Huangqi in the treatment of DN, this article explored the potential targets and signaling pathways of Astragalus-Chinese yam-Salvia miltiorrhiza in the intervention of diabetic nephropathy (DN), based on GEO database combined with network pharmacology and molecular docking. The relevant chips of DN were searched by GEO database, and the chemical active substances and corresponding targets in Astragali Radix, Dioscoreae Rhizoma and Salviae Miltiorrhizae Radix et Rhizoma were screened by TCMSP database, and the intersection with differentially expressed genes was taken. Then, the PPI network diagram was constructed by String database and Cytoscape 3.9.0 software respectively, and the gene GO and KEGG enrichment analysis were carried out by R language, and the above research results were verified by molecular docking. The results of molecular docking showed that the receptors IL6 and IL1 B could stably bind and interact with quercetin, diosgenin and luteolin. Thus, the active components such as quercetin, luteolin and diosgenin of Astragalus-Dioscorea-Salvia miltiorrhiza play a therapeutic role in DN by acting on IL6, IL1 B, MM9 and other targets, regulating AGE-RAGE and other signaling pathways.