化学发光
鲁米诺
肉眼
发光
生物传感器
化学
能量转移
荧光
电化学发光
费斯特共振能量转移
纳米技术
光化学
细菌
发光测量
检测点注意事项
材料科学
检出限
光电子学
色谱法
光学
生物
物理
分子物理学
免疫学
遗传学
作者
H. Wu,Yuan Fang,Lei Tian,Xin Liu,Xiao Zhou,Xiying Chen,Gao Hong,Haijuan Qin,Yaqing Liu
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2023-08-08
卷期号:8 (8): 3205-3214
被引量:3
标识
DOI:10.1021/acssensors.3c00918
摘要
Pathogenic bacteria are widely distributed in diverse environments and significantly threaten human health. Point-of-care testing (POCT) is a valuable way for early warnings of bacteria threat. Herein, a chemiluminescence (CL)-based ratiometric sensing platform was constructed for sensitive POCT of bacteria according to a newly designed aggregation-induced emission (AIE) molecule. The new AIE molecule presents oxidase-like properties (named as AIEzyme) and can trigger long persistent CL of luminol (LUM) with strong intensity in the absence of H2O2. The CL emission can be monitored with the naked eye for over 2 h. The emission mechanism is explored and may be attributed to the persistent reactive oxygen species generation of the AIEzyme according to the cyclic energy transfer between the AIEzyme and luminol, which catalyzes CL of luminol. Based on the CL resonance energy transfer mechanism, an afterglow luminescence system is further developed, which is used to construct a ratiometric biosensor for detection of pathogenic bacteria. With a homemade holder as a detection room and a smartphone as an analyzer, the portable biosensing platform is used for quantitative POCT of bacteria in real samples with good recovery. The detection is free of H2O2 and an external excitation source, which not only simplifies the operation but reduces interference. Specifically, the long persistent luminescence and the ratiometric strategy can significantly improve accuracy, providing an instructive way for point-of-need analysis, for example, SARS-CoV-2 detection and bioimaging analysis.
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