Soybean Agglutinin Induced Apoptotic Effects by Down‐Regulating ANXA2 Through FAK Pathway in IPEC‐J2 Cells

ABSTRACT Soybean agglutinin (SBA) is an anti‐nutritional factor in soybean, possesses toxic effects by binding to intestinal epithelial cells, and finally interferes the digestion and absorption of nutrients in humans and animals. Annexin A2 (ANXA2) is one of the SBA‐specific binding proteins in intestinal epithelial cells and participates in multiple cellular biological processes. However, whether SBA affects apoptosis through ANXA2 and its apoptosis‐related pathway remains unclear. IPEC‐J2 is an ideal model to study human intestinal health. Therefore, this study aims to investigate the effects of ANXA2 on SBA‐induced intestinal epithelial cell apoptosis and the related pathway mechanism using IPEC‐J2 as a cell model. The results showed that SBA induced the apoptosis through FAK signal pathway and decreased the gene and protein expressions of ANXA2 in IPEC‐J2. The expression of ANXA2 protein had a negative correlation with the apoptosis rates, and a positive correlation with the expression of FAK protein and FAK pathway downstream proteins. In conclusion, SBA induced apoptosis of IPEC‐J2 cells by downregulating the expression of ANXA2, which activated the FAK pathway. These findings highlight the toxic mechanism of SBA, which will provide basis for studying the toxicity mechanisms of other food‐derived anti‐nutrients and provide a new perspective for human gastrointestinal health and related cancer treatment.