Advanced strategies and recent food-related applications of cell surface display in Saccharomyces cerevisiae

Yeast surface display has emerged as a versatile whole-cell biocatalyst platform that enables the immobilization of recombinant proteins on the surface of Saccharomyces cerevisiae , eliminating the need for enzyme purification while improving catalytic efficiency, operational stability, and catalyst reusability. Recent advances in genetic engineering, synthetic biology, and high-throughput optimization have substantially enhanced display performance through the optimization of host strains, promoters, signal peptides, anchor proteins, linker design, and cell wall engineering. These developments have expanded applications in food processing, functional food ingredient production, food safety, and sustainable bioprocessing while facilitating catalyst reuse and simplified downstream processing. Despite these advances, industrial implementation remains limited by challenges associated with large-scale production, process integration, regulatory compliance, and techno-economic feasibility. This review summarizes recent advances in yeast surface display technology, with particular emphasis on engineering strategies, emerging food applications, industrial implementation, and future perspectives for sustainable food biomanufacturing.