Interleukin‐12 Signaling Promotes Podocyte Senescence in Lupus Nephritis

Objective Podocyte injury drives proteinuria and disease progression in lupus nephritis (LN), yet the mechanisms underlying podocyte dysfunction remain incompletely understood. Methods Sera samples from 39 patients with systemic lupus erythematosus, including 15 patients with LN and 13 healthy controls, were collected. Levels of interleukin‐12 (IL‐12) were measured by enzyme‐linked immunosorbent assay. Expression of IL‐12 receptors (IL‐12Rs) was assessed by immunofluorescence staining. Immortalized human podocyte cell line was used for in vitro study. The podocyte conditional knockout of Il12rb1 mice including B6. Il12rb1 fl/fl Nphs2 cre mice and B6.lpr. Il12rb1 fl/fl Nphs2 cre mice was generated. Accelerated lupus model and nephrotoxic nephritis model were induced. B6. Il12rb1 fl/fl and B6.lpr. Il12rb1 fl/fl mice were used as control. Results Serum levels of IL‐12 were elevated in patients with LN ( P = 0.006) and in mice who were lupus prone ( P = 0.041). Expression of the IL‐12R was increased in podocytes in both human and murine LN. IL‐12 stimulation induced the phosphorylation of calcium/calmodulin‐dependent protein kinase 4 and STAT4, causing mitochondrial dysfunction and increased reactive oxygen species accumulation, leading to accelerated podocyte senescence, senescence‐associated secretory phenotype, and cytoskeleton disorganization. Podocyte‐specific deletion of Il12rb1 attenuated podocyte senescence, ameliorated glomerular injury, and reduced proteinuria in both mice who were lupus prone and normal mice injected with a nephrotoxic serum. Conclusion Our findings identify IL‐12 signaling as an important mediator of podocyte senescence in LN and suggest that targeting the IL‐12–IL‐12R axis may offer future therapeutic approaches to preserve podocytes and attenuate LN. image