结合珠蛋白
结直肠癌
蛋白质组学
粪便
生物标志物
化学
蛋白质组
鸟枪蛋白质组学
血红素
血液蛋白质类
癌症
癌症研究
生物
免疫学
生物化学
微生物学
基因
酶
遗传学
血红素
作者
Ang C,Julie Rothacker,Heather Patsiouras,Antony W. Burgess,Edouard C. Nice
标识
DOI:10.1016/j.chroma.2009.10.007
摘要
Tumor related products shed into the feces offer a potential source of biomarkers for the detection of colorectal cancer (CRC). Using SDS-PAGE followed by nanoflow reversed-phased LC–MS/MS to analyse fecal samples from ApcMin/+ mice (that develop spontaneous multiple intestinal neoplasia with age) we have identified 336 proteins (115 proteins of murine origin, 201 from fecal bacteria, 18 associated with food intake and 2 of apparent parasitic origin). 75% of the murine proteins identified in this study are predicted to be extracellular or associated with the cell plasma membrane. Of these proteins, a number of the murine homologues of colorectal cancer associated proteins (CCAP) such as hemoglobin, haptoglobin, hemopexin, alpha-2-macroglobulin and cadherin-17 have been identified, demonstrating the potential of fecal proteomics for detecting potential biomarkers and paving the way for subsequent MS/MS based biomarker studies on similar human samples.
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