颊囊
细胞培养
癌
生物
抗原
膀胱
病理
体外
上皮样细胞
仓鼠
癌症研究
分子生物学
免疫学
内科学
医学
免疫组织化学
生物化学
遗传学
作者
Jan Bubenı́k,Magdalena Barešová,V. Viklický,J Jakoubková,H Sainerová,Judith Donner
标识
DOI:10.1002/ijc.2910110327
摘要
Abstract A cell line derived from human urinary bladder carcinoma, designated T24, was established in vitro. The growth of T24 cells in tissue culture was characterized by a disorderly pattern of growth in one or more layers and by mixed epithelioid‐fibroblastoid morphology. The generation time of T24 cells was 19 h. The cells had a hypotetraploid stemline with marker chromosomes. The malignant character of the line was verified by inoculation of cell suspension into hamster cheek pouch. The presence of tumour‐specific antigen (TSA) in T24 cells was demonstrated by a micromodification of the cytotoxicity test with autochthonous leukocytes and serum from the donor of the tumour. The TSA was also well detectable by the reaction with allogeneic leukocytes from tumour‐bearing patients and persisted during a 30‐month culture period. The permanent presence of TSA in this carcinoma cell line suggests that the TSA is a genetically determined characteristic of T24 cells.
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