单元格信封
抗菌剂
抗菌肽
生物物理学
细菌细胞结构
化学
膜
铜绿假单胞菌
细菌
原子力显微镜
纳米技术
材料科学
生物化学
大肠杆菌
生物
基因
有机化学
遗传学
作者
Shun Lu,Grant Walters,Richard Parg,John Dutcher
出处
期刊:Soft Matter
[The Royal Society of Chemistry]
日期:2014-01-01
卷期号:10 (11): 1806-1806
被引量:23
摘要
The effectiveness of antimicrobial compounds can be easily screened, however their mechanism of action is much more difficult to determine. Many compounds act by compromising the mechanical integrity of the bacterial cell envelope, and our study introduces an AFM-based creep deformation technique to evaluate changes in the time-dependent mechanical properties of Pseudomonas aeruginosa PAO1 bacterial cells upon exposure to two different but structurally related antimicrobial peptides. We observed a distinctive signature for the loss of integrity of the bacterial cell envelope following exposure to the peptides. Measurements performed before and after exposure, as well as time-resolved measurements and those performed at different concentrations, revealed large changes to the viscoelastic parameters that are consistent with differences in the membrane permeabilizing effects of the peptides. The AFM creep deformation measurement provides new, unique insight into the kinetics and mechanism of action of antimicrobial peptides on bacteria.
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